Scott Lab: Recent Highlights

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A Very Fast Minimal Hammerhead RNA

Sara M. O'Rourke, William Estell and William G. Scott

Minimal Hammerhead Ribozymes with Uncompromised Catalytic Activity.

Journal of Molecular Biology 427: 2340–2347 (2015). 10.1016/j.jmb.2015.05.005
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Supplemental
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G-Quadruplex RNA Structures

Alastair C. Fyfe, Pete W. Dunten, Monika M. Martick and William G. Scott

Structural Variations and Solvent Structure of r(UGGGGU) Quadruplexes Stabilized by Sr2+ Ions.

Journal of Molecular Biology 427: 2205-2219 (2015).
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25861762 image 4RKV
4RJ1
4RNE
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The Case for 2',3'-Cyclic Phosphates

William G. Scott, Abraham Szöke, Josh Blaustein, Sara M. O'Rourke and Michael P. Robertson

RNA Catalysis, Thermodynamics and the Origin of Life.

Life 4: 131-141 (2014). doi:10.3390/life4020131
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General or Specific Acid-Base Catalysis?

Eric P. Schultz, E. Vazquez, and William G. Scott

Structural and catalytic effects of an invariant purine substitution in the hammerhead ribozyme: implications for the mechanism of acid - base catalysis.

Acta Crystallographica Section D: Biological Crystallography D70: 2256-2263 (2014).
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25195740 image
3ZD4
3ZD5
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The Highest-Resolution Ribozyme Structure

Michael Anderson, Eric P. Schultz, Monika Martick and William G. Scott

Active-Site Monovalent Cations Revealed in a 1.55 Å Resolution Hammerhead Ribozyme Structure.

Journal of Molecular Biology 425: 3790–3798 (2013).
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23711504 image
3ZP8
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Do We Really Need Heavy Atom Derivatives?

Michael P. Robertson, Young-In Chi and William G. Scott

Solving novel RNA structures using only secondary structural fragments.

Methods. (2010) Jun 9.
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COOT

Emsley P, Lohkamp B, Scott WG, Cowtan K.

Features and development of Coot.

Acta Crystallogr D66:486-501 (2010).
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Antibody Interactions with HIV gp41 Variants

Sara M. O'Rourke, Becky Schweighardt, William G. Scott, Terri Wrin, Dora P. A. J. Fonseca, Faruk Sinangil, and Phillip W. Berman

Ring Structure in the gp41 Trimer of Human Immunodeficiency Virus Type 1 That Modulates Sensitivity and Resistance to Broadly Neutralizing Antibodies.

Journal of Virology83: 7728-7738 (2009).
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Class III Hammerhead Ribozyme Structures

Young-In Chi, Monika Martick, Rosalind Kim, William G. Scott and Sung-Hou Kim

Capturing Hammerhead Ribozyme Structures in Action by Modulating General Base Catalysis.

PLoS Biology 6(9): e234 (2008). doi:10.1371/journal.pbio.0060234
Paper
Accompanying commentary
18834200 image 2QUS (uncleaved)

2QUW (cleaved)
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3'-UTR-embedded Hammerhead RNAs that Regulate Gene Expression in Mammals

Monika Martick, Lucas H. Horan, Harry F. Noller & William G. Scott

A discontinuous hammerhead ribozyme embedded in a mammalian messenger RNA

Nature 454: 899-902 (2008).
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UCSC Press Release
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A Glimpse of Biology's First Enzyme

Michael P. Robertson and William G. Scott

The Structural Basis of Ribozyme-Catalyzed RNA Assembly

Science 315: 1549-1553 (2007).
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Perspective
Supplemental
17363667 image 2OIU
hammerhead ribozyme

The Full-Length Catalytically Active Hammerhead RNA Structure

Monika Martick and William G. Scott

Tertiary Contacts Distant from the Active Site Prime a Ribozyme for Catalysis

Cell 126: 309-320 (2006).
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Commentary
Supplemental
16859740 image 2GOZ
s2m RNA

RNase E

Anastasia J. Callaghan, Maria Jose Marcaida, Jonathan A. Stead, Kenneth J. McDowall, William G. Scott and Ben F. Luisi

Structure of E. coli RNase E catalytic domain and its implications for RNA turnover and processing.

Nature 437: 1187-1191 (2005).
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16237448 image 2C0B
2BX2
2C4R
s2m RNA

A Structural Element in the SARS Virus 3'-UTR

M. P. Robertson, H. Igel, R. Baertsch, D. Haussler, M. Ares, Jr., and W. G. Scott

The Structure of a Rigorously Conserved RNA Element within the SARS Virus Genome

Public Library of Science: Biology 3(1): e5. (2004).
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15630477 image 1XJR

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